stripping and western blot?

D.K. dk at no.email.thankstospam.net
Wed Sep 4 01:04:53 EST 2002


"Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote:
>
>I personally don't think this thread should steer away from the initial
>topic though. I was personally happy to learn that stripping can be done
>with acids, and wonder why, if it works so good, few labs know about this
>method and instead prefer stinking up everything with bME.
>

The usual stuff, I think - always following a given "protocol" and never
wanting to change what works. It's like Towbin buffer for blotting -
almost always the worst choice but is used in 95% cases. 

Seriously, try 0.1 M HCl or AcOOH - I am almost certain bet it will 
work fine.  

DK



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