best fusion tag for protein purification?

[Philipp Wechner]

davisdo at missouri.edu schrieb:

> Hello
>
> I am looking for the best (on average) affinity tag for efficient purification of recombinant proteins expressed in E. coli. Am using one of the pET series of expression vectors if that helps.

In my opinion the 6xHis-Tag ist best for purification.

>
>
> Also, does anyone know a superior bacterial strain for minimizing loss of recombinant protein to inclusion bodies?

I do not know a strain, but a vector. The thioredoxin-tag of the pET32 plasmids increases solubility of your protein and reduces the formation of inclusion bodies this way.

>
>
> Thanks, Doug
>
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
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