Acetone precipitation

vvjrp at yahoo.com vvjrp at yahoo.com
Wed Sep 11 01:23:23 EST 2002


Dear Vayuputra,
I basically did not understand whether you are interested in industrial scaleup or just in an analytical scale. The problem you are facing can be solved by both the ammonium sulfate precipitation or with the help of 100% ethanol.  If your objective is only to run an SDS-PAGE Gel then concentrate your samples using 90% ethanol.  Never even you keep your proteins at -80 degrees.Rather -20 or even 4 degress with EDTA is compatible.
Regards,
Dr.Kiran Kumar

http://biowww.net/mynews/tree.php?group_name=bionet.molbio.proteins&begin=0



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