Protein Electrophoresis

vvjrp at vvjrp at
Wed Sep 11 02:59:26 EST 2002

Dear Katrin
How much % of gel you are using. Plz check your Acrylamide and find out the % of it to be used.  This may solve your problem.  There is no problem for you if you load membrane proteins or whole cell extracts or even inclusion bodies.Maintain costant voltage while running a gel. Use fresh running buffer.
kiran kumar velpula
Katrin Westphal wrote:

> Hi,
> I have problems with my SDS-PAGE: The bands grow wider the further they
> have run and the resolution grows bad: bands at the the top seem to be
> ok, while those at the bottom are blurred and much bigger than the ones
> above.
> My samples are bacterial membrane proteins or whole cell extracts in
> sodiumphosphate buffer.

> Thanks in advance for any help!

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