alkylation, #2

chuck langton at
Fri Sep 13 21:33:07 EST 2002

Does anyone know of a good cysteine alkylation reagent that doesnt
result in a modification of 58 daltons?  i am looking for a
carboxymethylation on a cysteine (using esi ms/ms), and want to
alkylate the rest of the cysteines with a reagent with an MW that is
significantly different.  by the way, I tried NEM and didnt get a
great reaction apparently, so are there any other reagents people use
with mass spec? or what conditions do people use for NEM alkylation?

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