hlp_to_avoid_precipitation

danielak at intra.niddk.nih.gov danielak at intra.niddk.nih.gov
Tue Sep 17 14:00:04 EST 2002


Barak Akabayov wrote:

> Dear all:
> I have a protein 50 kD with certain pi.

>  Normally it is prepared within Hepes buffer pH 6.8 and ionic strength of
> 200mM (NaCl), unfortunately when I concentrate the protein after
> purification (above 10uM) it precipitate. How can I avoid it?





> Much appreciate any help

> Barak

>  barak_akabayov at yahoo.com

Try different buffers - once I was not able to dissolve a protein after lyophylisation in 0.05M phosphate buffer pH7, which I was using during whole purification procedure, but protein was dissolved in 0.05M ammonium bicarbonate without any problem up to concentration 20mg/ml



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