CAPS or Tris-Gly?

D.K. dk at no.email.thankstospam.net
Fri Sep 20 07:20:47 EST 2002


In article <vb9a9.99185$n04.2744733 at twister2.libero.it>, "News" <ulissec at libero.it> wrote:
>Are there any differences in the efficiency between CAPS and Tris-Gly/MetOH
>buffers for Western blotting?
>

Because of higher pH CAPS works better for very big and/or basic proteins.
Cheap stuff like carbonate and borate can be used very successfully 
in place of both of them. 

DK





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