Get problems in overexpression protein in E.coli
junk at hgmp.mrc.ac.uk
Sat Apr 5 05:20:54 EST 2003
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<20030401202908.16981.qmail at ww02.hostica.com> on Tue, 1 Apr 2003,
yourslynn at hotmail.com penned the following literary masterpiece:
>Hellow!I am overexpressing GST-fused protein in PET-41a vector in BL21(pLysS)host strain now.I have totally five proteins for overexpression.But
>only two get overexpressed.I tried the different incubation temperature and time,and with detergent addition on the other three,but failed.I have tested
>the plasmid stability,and find it's stable.So what's going on in my work?
Presumably you have sequenced and confirmed that what you have is
What is the source of the proteins, eubacterial, mammalian etc.?
Could the ones not expressing be using rare codons by E.coli codon
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