Problem with concentrating His Tag protein

[Louis Hom]

I'm not sure what volumes you're working with, but it may make sense to 
dialyze against some low ionic strength buffer for a short time before 
concentrating to avoid the problems DK mentioned.
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______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/