Problem with concentrating His Tag protein
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Tue Apr 29 16:26:33 EST 2003
In article <b8mjij$i2d$1 at agate.berkeley.edu>, lhom at OCF.Berkeley.EDU (Louis Hom) wrote:
>I'm not sure what volumes you're working with, but it may make sense to
>dialyze against some low ionic strength buffer for a short time before
>concentrating to avoid the problems DK mentioned.
With some proteins though, exactly opposite (higher salt
concentration) is needed to keep them in solution.
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