SDR kinetics

Nikolai Scherbak nikolai.scherbak at nat.oru.se
Wed Apr 30 09:38:16 EST 2003


Dear colleagues!
I am a doctoral student and doing kinetic studies on one NADH-dependent
enzyme that belongs to the SDR-family.
We are measuring activity using spectrophotometry at 340 nm. Activity curve
shows a rapid decrease of absorption in the very beginning (during approx.
10 seconds), then decrease dramatically slows but continues during next
approx 15 min of measurement.
The slope of this two parts of the curve both depends on concentration of
the substrate or enzyme.
Which slope should I use in my calculations of Vmax?

Thanks for your help,
Nikolai

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