Protein with 13 Prolines

Frank Küster frank at kuesterei.ch
Tue Dec 2 05:04:44 EST 2003


senmehmetsen at yahoo.com (Mehmet Sen) schrieb:

> I have a protein, about 26 kDa, and there are 13 prolines. It is
> getting expressed as inclusion bodies as attached to His-tag, but I
> could not get refolding of this stubborn protein yet. 

Have you tried to use PPIase?

> There are also 4
> cysteine, making s-s bonds. 

Hm, and possibly PDI with the right GSH-GSSG cocktail.

Bye, Frank
-- 
Bitte daran denken: Auch die Kaffeekasse hat manchmal Hunger.



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