Protein with 13 Prolines
frank at kuesterei.ch
Tue Dec 2 05:04:44 EST 2003
senmehmetsen at yahoo.com (Mehmet Sen) schrieb:
> I have a protein, about 26 kDa, and there are 13 prolines. It is
> getting expressed as inclusion bodies as attached to His-tag, but I
> could not get refolding of this stubborn protein yet.
Have you tried to use PPIase?
> There are also 4
> cysteine, making s-s bonds.
Hm, and possibly PDI with the right GSH-GSSG cocktail.
Bitte daran denken: Auch die Kaffeekasse hat manchmal Hunger.
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