Immunoprecipitate of big proteins

zhang184 at umn.edu zhang184 at umn.edu
Wed Dec 3 15:32:23 EST 2003


My antibodies work well in western blot to detect the 120kD proteins. But when I IP and blot the 
proteins, it seems not efficiently and also it's not good for histostaining. Can someone give me 
suggestings to efficiently pull dowm complexes with different lysis buffer. Thanks!


http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0



More information about the Proteins mailing list