Immunoprecipitate of big proteins

zhang184 at zhang184 at
Wed Dec 3 15:32:23 EST 2003

My antibodies work well in western blot to detect the 120kD proteins. But when I IP and blot the 
proteins, it seems not efficiently and also it's not good for histostaining. Can someone give me 
suggestings to efficiently pull dowm complexes with different lysis buffer. Thanks!

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