2D electrophoresis

Tom Anderson ucgatan at socrates-a.ucl.ac.uk
Thu Dec 4 09:27:34 EST 2003


On Tue, 2 Dec 2003, Tom Anderson wrote:

> On Thu, 27 Nov 2003, olivier michel wrote:
>
> > I am a beginner in 2D gel and i am facing the problem of long-term
> > storage of my gels for further digestion/analysis.
>
> How long-term? After fixing, staining and scanning, we used to seal
> them in plastic bags (actually, two bags, one inside the other, in
> case of leaks) using a heat-sealer, along with a splash of fixative
> (25% acetic acid in methanol),

Sorry, that should be 40% ethanol and 10% acetic acid in water. The
acetatic acid-methanol one is for cytology!

tom

-- 
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL




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