a question about gene expression in plasmids

Nick Theodorakis nick_theodorakis at hotmail.com
Sun Dec 14 21:09:27 EST 2003


On 14 Dec 2003 13:45:21 -0800, dikla_dar at walla.co.il (diki) wrote:

>hi everybody!
>my question is: in the plasmid pBR322, 

Wow! Blast from the past.!

>if I replace the whole gene for
>resistance to amp. in a certain insert, is there a good chance that
>the new gene will be expressed as a protein?

Depends. You got an E coli promoter on that insert?

> what are the
>circumstances in which it will happen? thank u very much for
>answering, diki.

There are a lot better vectors these days to express proteins in coli.
Or is this an exam question?


Nick

-- 
Nick Theodorakis
nick_theodorakis at hotmail.com
nicholas_theodorakis [at] urmc [dot] rochester [dot] edu 



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