a question about gene expression in plasmids
PC at no.email.sorry
Tue Dec 16 22:00:45 EST 2003
Frank Küster wrote:
> dikla_dar at walla.co.il (diki) schrieb:
>>my question is: in the plasmid pBR322, if I replace the whole gene for
>>resistance to amp. in a certain insert, is there a good chance that
>>the new gene will be expressed as a protein?
> One point that has not yet been mentioned:
> What will happen to the cells transformed with the plasmid if there is
> no longer an amp resistance on it?
grow the bags in tetracycline
If you adjust your growth conditions
> so that the cells still can grow, will the cells keep a high copy number
> of your plasmid? What results will that have for your expression level?
> Bye, Frank
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