is beta-galactosidase a glycoprotein?

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Thu Feb 27 21:20:00 EST 2003


Bacterial b-gal not glycosylated. Alternatively look for any large protein
or fusion protein expressed in E.coli - these will likely not be
glycosylated.

A.G.E.
"Justin Kemp" <justin.news.invalid at web2news.net> wrote in message
news:16882N281 at web2news.com...
> I am looking for a high molecular weight protein above 100 kDa (excl.
> myosin heavy chain or phosphorylase B) to act as a negative marker for a
> glycoprotein stain. I have been told that beta-galactosidase may be
> suitable. Can anyone tell me if it is indeed devoid of carbohydrate?
> Any help much appreciated,
> Justin (PhD student).
>
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