Endonuclease

Tom Knight tk at suspiria.ai.mit.edu
Fri Jan 3 18:42:44 EST 2003


Invitrogen uses the ccdB gene which is toxic in E. coli as a way of
doing positive selection of insertions in its cloning systems.  If you
look at their vector sequences you can find the insertion, and you
could easily get a copy of the gene from any one of a number of their
vectors.  I think they even sell a resistant strain of coli, so that
you can grow it.





More information about the Proteins mailing list