plotting enzyme activity

adeboer at chem.vu.nl adeboer at chem.vu.nl
Mon Jan 6 10:08:45 EST 2003


Hello,

I am an analytical chemist, combining enzymes with analytical instruments. Now I have a question.
If I will obtain Km and Vmax out of a michealis-menten plot, which type of plot is the best to use, I mean: lineweaver-burk, eadie-hofstee, hanes-woolf, direct linear...? 


I look forward to hearing from you
A. de Boer
Vrije Universiteit Amsterdam
The Netherlands

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