plotting enzyme activity

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Mon Jan 6 19:49:13 EST 2003


These days I would use constrained multivariable fit-to-data (using an
appropriate starting model guess, but allowing for expansion into more
complex models). Linear plots are OK for reactions that approximate
linearity, however much better accuracy can be achieved with nonlinear
fitting.

A.G.E.
<adeboer at chem.vu.nl> wrote in message
news:20030106150845.20429.qmail at ww02.hostica.com...
> Hello,
>
> I am an analytical chemist, combining enzymes with analytical instruments.
Now I have a question.
> If I will obtain Km and Vmax out of a michealis-menten plot, which type of
plot is the best to use, I mean: lineweaver-burk, eadie-hofstee,
hanes-woolf, direct linear...?
>
>
> I look forward to hearing from you
> A. de Boer
> Vrije Universiteit Amsterdam
> The Netherlands
>
>
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