To separate protein from Ni+2/EDTA

Debashis Mukhopadhyay debmukh at
Tue Jan 14 18:28:03 EST 2003


I am using Ni affinity column to isolate my protein which came out
successfully with Imidazole. However, I found a lot (~100 mg) of protein
still sticking to the column and I eluted them out by stripping off the
Ni+2 from the column using EDTA. So, this solution now contains my protein
+ (may be some contaminant) + Ni and EDTA and is colored blue (as
expected). At this point I wonder if there is any way / standard practice
to separate the protein from Ni+2, so that I can re-utilize it.

Any help would be appreciated.

                 Debashis Mukhopadhyay, Ph.D.
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