Help on small M.W. protein Western blot

Jay H. hj at digivista.com
Wed Jan 15 13:08:31 EST 2003


Hi everyone, I've been trying to prove the presence of a ~11KDa protein in
transgenic N.b. plants (35S promoter driven).  The positive control (E. coli
expressed) worked fine but I couldn't detect anything from plant leaf
tissue:(  Does anyone here have related experience?  Or any good protein
extraction method to recommend?  Thanks a lot!

Jay





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