cloud point separation

Mark Bowen mboNOT at slac.stanford.edu
Wed Jan 22 05:58:37 EST 2003


I am interested in using detergent cloud-point phase-separation to 
select for membrane proteins in a mixture of semi-purified proteins.  I 
want to remove soluble cleavage products from full-length membrane 
proteins.  Does anyone have a reliable method they could share?  Ideally 
I'd like to work in the cold and with some salt.  

-- 
Mark Bowen






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