cloud point separation
legatek at mcmail.cis.mcmaster.ca
Wed Jan 22 16:43:39 EST 2003
On Wed, 22 Jan 2003, Mark Bowen wrote:
> I am interested in using detergent cloud-point phase-separation to
> select for membrane proteins in a mixture of semi-purified proteins. I
> want to remove soluble cleavage products from full-length membrane
> proteins. Does anyone have a reliable method they could share? Ideally
> I'd like to work in the cold and with some salt.
Some might suggest you try the Triton X-114 phase separation assay (J.
Biol. Chem. (1981)256:1604-1607), even though you can't do it in the cold
(TX114 reaches its cloud point at around 20C). However, it is generally
accepted these days that this method is unreliable.
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legatek at mcmaster.ca Kyle Legate legatek at hotmail.com
Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
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