cloud point separation

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Wed Jan 22 16:43:39 EST 2003


On Wed, 22 Jan 2003, Mark Bowen wrote:

> I am interested in using detergent cloud-point phase-separation to
> select for membrane proteins in a mixture of semi-purified proteins.  I
> want to remove soluble cleavage products from full-length membrane
> proteins.  Does anyone have a reliable method they could share?  Ideally
> I'd like to work in the cold and with some salt.
>
Some might suggest you try the Triton X-114 phase separation assay (J.
Biol. Chem. (1981)256:1604-1607), even though you can't do it in the cold
(TX114 reaches its cloud point at around 20C). However, it is generally
accepted these days that this method is unreliable.

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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