Native PAGE

senthilkumarsr at senthilkumarsr at
Tue Jan 28 10:18:25 EST 2003

My protein MW 13 KDa runs smoothly in 12% native PAGE gel and it is spotted just above the dye front. When the same sample is loaded in 15 native PAGE gel, it stops in the middle of the gel while the dye front and the marker moves finely. Except the gel % all the conditions are the same. What could be the reason for the partial mobility of the protein in 15% native PAGE gel .

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