meb at meb.emb
Tue Jan 28 12:55:54 EST 2003
your reply couldn't have been more helpful. have you actually used phage to
induce expression or do you just need to reply to everything?
I am curious about how well phage induction of vectors like the pet series
works for expression of poorly tolerated constructs. It is not often used
anymore. I have indeed tried google and pubmed (which was a truly inspired
suggestion), but keywords like phage and induction pull up too many hits to
zero in on anything approaching a useful protocol.
Are there particular papers amongst the umpteen you allude to that could be
"Duncan Clark" <firstname.lastname@example.org> wrote in message
> Historians believe that in newspost <b146vm$2vk$1 at news.Stanford.EDU> on
> Mon, 27 Jan 2003, Mark Bowen <mboNOT at slac.stanford.edu> penned the
> following literary masterpiece:
> >Specifically introduction of T7 polymerase by phage. Does anyone have
> >any experience in this area?
> Umpteen old papers on T7 around.
> What do you actually want to know and have you tried Pubmed or Google
> etc. ?
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
> Duncan Clark
> GeneSys Ltd.
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