phage induction

Duncan Clark junk at hgmp.mrc.ac.uk
Wed Jan 29 05:26:23 EST 2003


Historians believe that in newspost <b16hhp$d92$1 at news.Stanford.EDU> on 
Tue, 28 Jan 2003, meb <meb at meb.emb> penned the following literary 
masterpiece:
>your reply couldn't have been more helpful.

Well that is your pig ignorance in not choosing to learn how to do a 
search properly and asking a pretty vague question. You then shoot down 
a genuine reply. If you had mentioned pET vectors all would have been 
revealed.

>have you actually used phage to
>induce expression

Yes. CE6. If you know about T7 RNA polymerase expression vectors you 
would know about Studier, Tabor or Richardson. A search on them could 
bring great rewards, especially as the system is I think licensed, if 
not patented.

>or do you just need to reply to everything?

Well given that I rarely, if ever, post this group (14x since 1993 
according to googlegroups) that is bollocks. I tend to post to the 
methods reagents newsgroup, where an archives search would show whether 
I talk crap or not. If you searched the archives of 
bionet.molbio.methds-reagnts00 you would find a lot of posts on CE6 
including three from me with the first one dating back to 1996.

>I am curious about how well phage induction of vectors like the pet series
>works for expression of poorly tolerated constructs.

Try Novagen, Stratagene plus a search for CE6 would fined the 
appropriate papers.
>

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



More information about the Proteins mailing list