SDS quantitation and removal with resins

Nick Holway nickholway at hotmail.com
Wed Jul 2 09:03:54 EST 2003


Scott Coutts <scott.coutts at med.monash.edu.au> wrote in message news:<3F024A95.40702 at med.monash.edu.au>...
> Hi!
> 
> Does anyone know of a way that I can assay for SDS concentration in a
> sample containing protein? I want to know how much detergent remains 
> after attempts at removal. I need to assay for regular (non-labelled) 
> SDS, by the way.

You could always attack the problem from a different angle and use the
SDS as a blank and then subtract its absorbance from the value with
the sample in it.

Nick



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