SDS quantitation and removal with resins

Scott Coutts scott.coutts at med.monash.edu.au
Thu Jul 3 19:30:21 EST 2003


oh, sorry.

It's sodium dodecyl sulphate (or sodium lauryl sulphate).

Joe 123 wrote:
> what is sds?  sodium dodecyl benzene sulfonate.  Just curious.
> 
> 
> 
> "Scott Coutts" <scott.coutts at med.monash.edu.au> wrote in message
> news:3F024A95.40702 at med.monash.edu.au...
> 
>>Hi!
>>
>>Does anyone know of a way that I can assay for SDS concentration in a
>>sample containing protein? I want to know how much detergent remains
>>after attempts at removal. I need to assay for regular (non-labelled)
>>SDS, by the way.
>>
>>Also, I'm thinking of removing the SDS from some protein samples using a
>>resin, perhaps Dowex 1-X2 or 1-X8 or similiar. I'm thinking of doing it
>>in a batch method and then spinning the resin out through a frit. Does
>>anybody have any comments about this? I.e. are there better resins for
>>this type of application? Better ways of going about it? Is this likely
>>to be more efficient than acetone or TCA precipitation (I don't care
>>about protein function or recovery of secondary/tertiary structure).
>>
>>Lastly, does anyone have any experience with Pierce's "SDS-Out"
>>precipitation reagent? Anybody know what's in it?
>>
>>
>>Thanks!
>>
>>
> 
> 
> 




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