SDS quantitation and removal with resins

Peter Sjödin peter.sjodin at chello.nospam.se
Tue Jul 8 06:56:49 EST 2003


"Scott Coutts" <scott.coutts at med.monash.edu.au> skrev i meddelandet
news:3F024A95.40702 at med.monash.edu.au...
> Hi!
>
> Does anyone know of a way that I can assay for SDS concentration in a
> sample containing protein? I want to know how much detergent remains
> after attempts at removal. I need to assay for regular (non-labelled)
> SDS, by the way.
>
> Also, I'm thinking of removing the SDS from some protein samples using a
> resin, perhaps Dowex 1-X2 or 1-X8 or similiar. I'm thinking of doing it
> in a batch method and then spinning the resin out through a frit. Does
> anybody have any comments about this? I.e. are there better resins for
> this type of application? Better ways of going about it? Is this likely
> to be more efficient than acetone or TCA precipitation (I don't care
> about protein function or recovery of secondary/tertiary structure).
>
> Lastly, does anyone have any experience with Pierce's "SDS-Out"
> precipitation reagent? Anybody know what's in it?
>
>
> Thanks!
>

Why not meassure the dynamic surface tension?
This will give you the koncentration of the SDS only and not any of the
protein.
Calibrate with known koncentration.
www.jenser.com and www.sensadyne.com and others knows a lot about this.

Best regards
Peter





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