SDS quantitation and removal with resins
coljmax at bigfoot.com
Tue Jul 8 08:31:50 EST 2003
Scott Coutts <scott.coutts at med.monash.edu.au> wrote in message news:<3F024A95.40702 at med.monash.edu.au>...
> Does anyone know of a way that I can assay for SDS concentration in a
> sample containing protein? I want to know how much detergent remains
> after attempts at removal. I need to assay for regular (non-labelled)
> SDS, by the way.
ASTM D1681-92(1997) Standard Test Method for Synthetic Anionic Active
Ingredient in Detergents by Cationic Titration Procedure (see last
page) http://www.techstreet.com/cgi-bin/detail?product_id=6966 will
give you total SDS. BDH sell the cationic titrant Hyamine 1622 0.004M
( which can be used instead of CTAB) and the dimidium
bromide-disulphine blue mixed indicator. The endpoint is very sharp
provided you are not color-blind!
> Also, I'm thinking of removing the SDS from some protein samples using a
> resin, perhaps Dowex 1-X2 or 1-X8 or similiar. I'm thinking of doing it
> in a batch method and then spinning the resin out through a frit. Does
> anybody have any comments about this? I.e. are there better resins for
> this type of application? Better ways of going about it? Is this likely
> to be more efficient than acetone or TCA precipitation (I don't care
> about protein function or recovery of secondary/tertiary structure).
> Lastly, does anyone have any experience with Pierce's "SDS-Out"
> precipitation reagent? Anybody know what's in it?
It could be a long chain quaternary ammonium compound such as the
cationic titrants mentioned above.
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