which Tag is best?

jdu at benaroyaresearch.org jdu at benaroyaresearch.org
Mon Jun 2 17:53:42 EST 2003


I want to put a tag at either end of a gene of interest, and then express this gene in Mouse T cells. Finally, I want to recover the expressed fusion protein with the monoclonal antibody against this tag. I do not want my fusion protein to be contaminated with other proteins from T cells (I mean the monoclonal antibody has negligible cross reactivity with other proteins from T cells). Which tag is the best for this purpose? Please help me make this decision? Thanks.


http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0



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