Blue Native page problems

Ben Long long at rsbs.anu.edu.au
Tue Jun 17 00:32:36 EST 2003


Hi all,

I am trying to run blue native gels of cyanobacterial thylakoid membrane
complexes and keep running into a problem with protein precipitation in the
gel.  I am extracting with 1.5 % dodecyl maltoside and running gels
according to the method of Schagger et al.  If anyone has developed new
methods or managed to trouble-shoot this sort of thing before I would be
interested to know how and what can be done to achieve nice band separation
and reduced precipitation.

Cheers,

Ben





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