Blue Native page problems
rkumar at bio1.rwth-aachen.de
Tue Jun 24 11:43:58 EST 2003
try 2.5% final conc. of dodecyl maltoside and spin the sample shortly
before loading on gel.
Ben Long wrote:
> Hi all,
> I am trying to run blue native gels of cyanobacterial thylakoid membrane
> complexes and keep running into a problem with protein precipitation in the
> gel. I am extracting with 1.5 % dodecyl maltoside and running gels
> according to the method of Schagger et al. If anyone has developed new
> methods or managed to trouble-shoot this sort of thing before I would be
> interested to know how and what can be done to achieve nice band separation
> and reduced precipitation.
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