Protein detection problem under reducing condition

Mauro Bongrazio mbon at zedat.fu-berlin.de
Wed Jun 25 06:46:15 EST 2003


Hallo everybody,
I have a home-made anti-peptide antibody which detects the target
protein under non-reducing but not under reducing (beta-mercapto)
conditions. Do you know why? I thought the contrary could be the
case
Thanks
Mauro

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