Lowry protein determination

James Wee jywee at charter.net
Thu Jun 26 11:21:24 EST 2003


"Charter News" <jywee at charter.net> wrote in message
news:vfidagolo8e2b7 at corp.supernews.com...
> Hello,
>
> I have a problem to construct a standard curve by using Bovine Serum
> Albumin. The standard curve is linear, but it intercepts y-axis at  0.248
> Abs (Beckman spectrometer). The sample values I want to compare with the
> standard curve are lower than the y-intercept value, e.g. 0.0987. Is that
> the problem with the BSA? Or there is something wrong with my sample
> readings?
>
> Any clue?
>
> Thanks,
> James
>
>
>
>





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