Protein detection problem under reducing condition

Mauro Bongrazio mbon at zedat.fu-berlin.de
Fri Jun 27 02:40:12 EST 2003


"
 reducing.." and "..non-reducing conditions 
" refer to the target protein and not
to the antibody. The Ab was used for immunoblot detection of target protein in cell
lysates resolved by SDS-PAGE under reducing (no signal) or non-reducing (signal)
conditions.
Mauro

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