35s labled gst fusion protein

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Mar 5 20:58:43 EST 2003


On Wed, 05 Mar 2003 09:59:33 +0100, Dr Engelbert Buxbaum
<engelbert_buxbaum at hotmail.com> wrote:

>fisher wrote:
>
>> If I want to get this kind of protein in E.coli, how to do it?
>> Does it need in vitro translation?
>
>
>Buy some Translabel (a mixture of 35S methionine and cysteine) and a
>sulfur-free medium (for mammalian cell culture these are readily
>available, I'm not sure for E. coli). Grow your bugs in an o/n culture,
>spin them down and wash with sulfur-free medium. 
...

Is TransLabel cheaper than [35S]sulfate? I thought that TransLabel was
made by incubating bugs in [35S]sulfate and then taking an amino acid
fraction.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu



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