Freeze-drying problem--Part 2

James Bassuk bassuk at u.washington.edu
Thu Mar 20 18:16:35 EST 2003


On 11 Mar 2003, Phil Harrison wrote:

> Worse news!  I tried redissolving the goo, including several spots on the
> side of the freeze-dryer flask, and it didn't go into solution.  All I got
> was a cloudy suspension.  Any thoughts?  Have I removed something (a
> carbohydrate--maybe a sugar alcohol) that was keeping the protein from
> becoming fully dehydrated?
>
> Thanks,
>
> Phil
>
> Phil Harrison
>
> USDA-Agricultural Research Service,
> Forage and Range Research Lab
> Utah State University,  UMC 6300
> Logan, UT 84322-6300
> Phone: 435-797-3209
> FAX: 435-797-3075
> e-mail: arsphys at cc.usu.edu
>
> ---
>

What is the buffer comnposition of your biogel column?
Have you tried dialyzing your column fractions against a volatile solvent
(like 0.1% acetic acid...BTW, what is the pI of your protein) prior to
lyo?

JIm




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