HIS TAG - Metal affinity column

Duncan Clark junk at hgmp.mrc.ac.uk
Fri Mar 21 13:03:17 EST 2003


Historians believe that in newspost 
<%iGea.6733$sK6.897661 at news20.bellglobal.com> on Fri, 21 Mar 2003, Kyle 
Legate <legatek at sympatico.ca> penned the following literary masterpiece:
> I have a His
>tagged protein that will bind to a Nickel column in the absense of
>imidazole, but the inclusion of even 5 mM imidazole in the binding buffer
>greatly reduces my yield.

Another thing to try is Copper as opposed to Nickel. We had a student 
maybe three years ago who found the APB chelating sepharose charged with 
Cu to be much better for the 5 or 6 proteins she played with, than Ni.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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