Seperation Techniques

pink_flamingo at mexico.com pink_flamingo at mexico.com
Sat Mar 22 19:13:20 EST 2003


Hi just wondering if anyone had some ideas for this 

Q/  You want to prepare a quantity of a protein from a biological sample.  The protein has a MW of about 45kD and a pI of 6.  It binds to the amino acid tyrosine but in the crude biological sample the protein is already saturdated with tyrosine, and the protein is unstable below its isoeletric point.  Outline a scheme, by which the protein could be purified by chromatographic means..including stationary phase, and conditions for elcution etc


http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0



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