DK at no.valid.address
Sun Mar 23 10:10:11 EST 2003
In article <20030323001320.18467.qmail at ww02.hostica.com>, pink_flamingo at mexico.com wrote:
>Hi just wondering if anyone had some ideas for this
>Q/ You want to prepare a quantity of a protein from a biological sample. The
> protein has a MW of about 45kD and a pI of 6. It binds to the amino acid
> tyrosine but in the crude biological sample the protein is already saturdated
> with tyrosine, and the protein is unstable below its isoeletric point.
> Outline a scheme, by which the protein could be purified by chromatographic
> means..including stationary phase, and conditions for elcution etc
This reads like a homework assignment. Poor question, too. It only makes
sense if rephrased as "outline ways by which the protein could be tried
to be purified by chromatographic means".
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