DPPIV purification

USF chihara at usfca.edu
Mon Mar 31 17:20:31 EST 2003


We are trying to purify DPPIV from drosophila using standard techniques 
from the lit. We are finding that after putting the homogenate of 
Membrane fraction through  a DEAE column, and concentrating we lose all 
the enzymatic activity.  We were able to concentrate after gel 
filtration, and there is activity in the fractions off the DEAE column. 
 Does anyone have any suggestions as to where to look for the problem?
Thanks.

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