Detect TAP tag

mj dreamwestus at yahoo.com
Thu May 22 18:16:43 EST 2003


Hi, all

I tagged a protein with TAP tag.  Now I tried to detect the fusion protein
by detecting the CBP(calmodulin binding peptide) part of the tag using
Western. Is there a CBP antibody commercially available??

I already tried to use biotinylated calmodulin to detect it, but the western
sucks, lots of background and I can't see my band.  Does anybody have any
suggestions?

Thanks! Help will be much appreciated!







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