4 basic questions regarding denatured protein and denaturing agent

lilyzhao_z at yahoo.com lilyzhao_z at yahoo.com
Thu May 22 20:47:15 EST 2003


Dear all, 

I have four basic questions regarding denatured protein and denaturing agent:

1. If a protein is denatured, will it's absorbance at 280 nm change?  If changes, what kind of change is it?

2. Do 6M Gu-HCl and 8M Urea have strong absorbance at 280 nm?

3. Will Gu-HCl react with bromophenol blue (which is used as dye in the sample buffer for SDS-PAGE) at high temperature?

4. Which one is a stronger denature agent, Gu-HCl or Urea?

Thank you in advance,

Lily


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