Problems with SDS-PAGE

Kerstin.Wechler at freenet.de Kerstin.Wechler at freenet.de
Mon May 26 07:33:25 EST 2003


For some time I have problems with the SDS PAGE. The proteins originate from lysierten human cells (MCF-7). Approx. 30 µg protein are loaded, on an 10 % acrylamide separation gel and electrophoresis runs with 200 V. My problem: the protein do not overflow into the separation gel and thus are not seperated. 


http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0



More information about the Proteins mailing list