Problems with SDS-PAGE

Kerstin.Wechler at Kerstin.Wechler at
Mon May 26 07:33:25 EST 2003

For some time I have problems with the SDS PAGE. The proteins originate from lysierten human cells (MCF-7). Approx. 30 µg protein are loaded, on an 10 % acrylamide separation gel and electrophoresis runs with 200 V. My problem: the protein do not overflow into the separation gel and thus are not seperated.

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