Help!!Separate a protein from 2-mercaptoethanol

EK anybody at uchicago.edu
Sat May 31 18:17:03 EST 2003


That falls under "dialysis".
EK

"SDA" <fa245812 at skynet.be> wrote in message
news:3ED85AD1.9050604 at skynet.be...
> wouldn't it be easier (and quicker) simply to do a buffer exchange with
> G-25 columns (PD-5 etc.?)
>
> EK wrote:
> > Dialysis, salt or solvent precipitation, ion exchange chromatography and
> > whatnot. However, nobody can guarantee that your protein can survive any
of
> > those. Do you know that BME is not critical to maintain your protein's
> > stability? I would start with dialysis as the gentlest method. Don't
tell me
> > there are no suitable membranes on the market. Nowadays you just need to
> > have money :-).
> > EK
> >
> > <nkokkoni at yahoo.com> wrote in message
> > news:20030528202149.11253.qmail at ww02.hostica.com...
> >
> >>I need to separate my 4000Da Protein from 2-mercaptoethanol. Can anyone
> >
> > suggest a procedure that will give me a pure protein with high yield?
> >
> >>
> >
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
> >
> >
>





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