running sds gel with guanidium

P.C. PC at no.email.sorry
Wed Nov 26 22:22:19 EST 2003


just curious: did you load the samples while they were still hot? (which 
is the most common way to deal with low solubility of Gu- or 
K-dodecylsulfate).

Peter

ss at nordicbioscience.com wrote:

> I tried to analyse by western-blot protein samples that I have treated with guanidium. But the proteins migrated in a smear, like if all the samples were degraded. Is there a problem to run an SDS page in presence of guanidium?
> 
> 
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0




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