running sds gel with guanidium

P.C. PC at
Wed Nov 26 22:22:19 EST 2003

just curious: did you load the samples while they were still hot? (which 
is the most common way to deal with low solubility of Gu- or 


ss at wrote:

> I tried to analyse by western-blot protein samples that I have treated with guanidium. But the proteins migrated in a smear, like if all the samples were degraded. Is there a problem to run an SDS page in presence of guanidium?

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