tissue homogenization

Aravind remove.this-aravind at charter.net
Sun Nov 30 11:57:20 EST 2003


I guess one could use "homogenizers" that one could buy (Bio Rad?),
sonication could work, buffer to use would depend on intended use of the
homogenized material.

--
..
Rolands G. Aravindan
rolands_aravindan at yahoo.com
__________________________
Scott Coutts wrote in message <3F435ED3.6060601 at med.monash.edu.au>...
>Oh, sorry, I forgot to mention something... I cant remember what the
>little eppy type mortar and pestles were called or who sold them!
>
>What about sonication?
>
>Scott Coutts wrote:
>> Hmm... dont know about the BEST way, but you can buy little 'mortar and
>> pestle' type things that fit exactly into the bottom of an eppendorf
>> tube. We used them a while back for crushing and homogenising individual
>> Drosophila flies.
>>
>> As for the solvent, well, I guess that depends on how you have your
>> ELISAs set up.
>>
>> julie.decock at med.kuleuven.ac.be wrote:
>>  >
>>
>>> what is the best method for homogenization of very small
>>
>>  > amounts (2-18 mg) of mammary tumor tissue for ELISA and
>>  > in which solvent should this be done?
>>
>>>
>>
>





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