protein folding on Ni-column
tatjana.schwabe at ruhr-uni-bochum.de
Thu Sep 11 12:59:12 EST 2003
Wow, this might be my chance! Yes, there is a reference I happen to
Schwabe, T. M., Gloddek, K., Schluesener, D. and Kruip, J., Purification
of recombinant BtpA and Ycf3, proteins involved in membrane protein
biogenesis in Synechocystis PCC 6803. J Chromatogr B Analyt Technol
Biomed Life Sci, 2003. 786(1-2): p. 45-59
Actually we did it the way DK suggested, by a gradient from 6M to 0M
urea at RT. It was easy for the first protein and gave very good
results, protein #2 didn't like it, but this one was more hydrophobic...
There is also an application manual from Amersham (should belong to
their ÄKTA series), which has a procedure about this.
richardfundgrueb at hotmail.com wrote:
> I want to refold a protein bound by His-tag to a Ni-column. Can anyone give me some references in which this has been previously done?
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