protein folding on Ni-column

Schwabe, TME tatjana.schwabe at ruhr-uni-bochum.de
Thu Sep 11 12:59:12 EST 2003


Wow, this might be my chance! Yes, there is a reference I happen to 
remember:
Schwabe, T. M., Gloddek, K., Schluesener, D. and Kruip, J., Purification 
of recombinant BtpA and Ycf3, proteins involved in membrane protein 
biogenesis in Synechocystis PCC 6803. J Chromatogr B Analyt Technol 
Biomed Life Sci, 2003. 786(1-2): p. 45-59

:-)

Actually we did it the way DK suggested, by a gradient from 6M to 0M 
urea at RT. It was easy for the first protein and gave very good 
results, protein #2 didn't like it, but this one was more hydrophobic...

There is also an application manual from Amersham (should belong to 
their ÄKTA series), which has a procedure about this.


Tatjana

richardfundgrueb at hotmail.com wrote:

> I want to refold a protein bound by His-tag to a Ni-column. Can anyone give me some references in which this has been previously done?
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0




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